Journal: Journal of Oncology

Article Title: Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling

doi: 10.1155/2022/3565118

Figure Lengend Snippet: The expression of MLLT10 could affect cell apoptosis in CRC. (a) Expression of MLLT10. (b), (c) The expressions of cleaved caspase 3, caspase 9, Bax, and Bcl-2 at the gene and protein levels. (d) The apoptosis rate of HCT116 cells. ∗ P < 0.05, one-way ANOVA.

Article Snippet: The antibodies used were as follows: anti- β -actin (1 : 5000, 66009-1-Ig, proteintech), anti-PCNA (1 : 2000, 10205-1-AP, proteintech), anti-Ki67 (1 : 1000, 27309-1-AP, proteintech), anti-cleaved caspase 3 (1 : 1000, 9664S, CST), anti-caspase 9 (1 : 500, bs-20773R, Bioss Antibodies), anti-Bax (1 : 1000, ab32503, abcam), anti-Bcl-2 (1 : 1000, 12789-1-AP, proteintech), anti-E-cadherin (1 : 1000, 20874-1-AP, proteintech), anti- β -catenin (1 : 1000, bs-1165R, Bioss Antibodies), anti- N-cadherin (1 : 2000, 22018-1-AP, proteintech), anti-vimentin (1 : 2000, 10366-1-AP, proteintech), anti-Snail (1 : 1000, 13099-1-AP, proteintech), anti-Slug (1 : 1000, #9585, CST), HRP goat anti-mouse IgG (1 : 5000, SA00001-1, proteintech), and HRP goat anti-rabbit IgG (1 : 6000, SA00001-2, proteintech).

Techniques: Expressing

Journal: Journal of Oncology

Article Title: Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling

doi: 10.1155/2022/3565118

Figure Lengend Snippet: Effects of Lnc-S100B-2 and MLLT10 in vivo . (a, b) Expression of Lnc-S100B-2 and MLLT10. (c, d) Tumor volume and mass. (e) Expression of caspase 3 by IHC. (f–k) The expression of PCNA, Ki67, caspase 9, Bax, and Bcl-2 at the gene and protein levels. ∗ P < 0.05 versus NC group, # P < 0.05 versus sh-MLLT10 group, and & P < 0.05 versus sh-Lnc-S100B-2 group, one-way ANOVA and two-way ANOVA.

Article Snippet: The antibodies used were as follows: anti- β -actin (1 : 5000, 66009-1-Ig, proteintech), anti-PCNA (1 : 2000, 10205-1-AP, proteintech), anti-Ki67 (1 : 1000, 27309-1-AP, proteintech), anti-cleaved caspase 3 (1 : 1000, 9664S, CST), anti-caspase 9 (1 : 500, bs-20773R, Bioss Antibodies), anti-Bax (1 : 1000, ab32503, abcam), anti-Bcl-2 (1 : 1000, 12789-1-AP, proteintech), anti-E-cadherin (1 : 1000, 20874-1-AP, proteintech), anti- β -catenin (1 : 1000, bs-1165R, Bioss Antibodies), anti- N-cadherin (1 : 2000, 22018-1-AP, proteintech), anti-vimentin (1 : 2000, 10366-1-AP, proteintech), anti-Snail (1 : 1000, 13099-1-AP, proteintech), anti-Slug (1 : 1000, #9585, CST), HRP goat anti-mouse IgG (1 : 5000, SA00001-1, proteintech), and HRP goat anti-rabbit IgG (1 : 6000, SA00001-2, proteintech).

Techniques: In Vivo, Expressing

Journal: Scientific Reports

Article Title: MG-132 reduces virus release in Bovine herpesvirus-1 infection

doi: 10.1038/s41598-017-13717-1

Figure Lengend Snippet: MG-132 inhibits BoHV-1-induced apoptosis in MDBK cells. ( A ) Cell lysate was prepared from mock-infected cells (lanes C), or infected cells, exposed or not to MG-132 and, for the indicated times, Western blot analysis was performed with an antibody which specifically recognized pro-caspase 9, cleaved caspase 9, pro-caspase 3, cleaved caspase 3, Bax, Bcl-2, Bcl-X L , PARP, cleaved PARP, p53, p-p53 or β-actin. β-actin was used as an internal loading control. Blots are representative of three separate experiments. ( B , C ) Bax/Bcl-2 and p-p53/p53 ratio were obtained by densitometry analysis of the relative blots shown in ( A ). Results are the mean ± S.E.M. of three separate experiments.

Article Snippet: The following antibodies, dissolved in 5% bovine serum albumin-TBST, were used: anti-caspase-3 PAb (1:1000) (Santa Cruz Biotechnology, sc-1225), anti-caspase-9 PAb (1:500) (Acris Antibodies, Inc., AP00004PU-N), anti-p53 MAb (1:1000) (Santa Cruz Biotechnology, sc-99), anti-phospho-p53 PAb (Ser315) (1:1000) (Cell Signaling, 2528), anti-PARP Mab (1:5000) (Biomol, BML-SA250-0050), anti-Bax Pab (1:1000) (Abcam, ab7977), anti-Bcl-2 Pab (1:1000) (Abcam, ab7973), anti-Bcl-X L Pab (1:1000) (Abcam, ab2568), polyclonal rabbit anti-bICP0 (a.a. 663–676) serum (1:800), kindly provided by Prof. M. Schwyzer (University of Zurich, Switzerland) , anti-LC3 PAb (Novus Biologicals, NB100-2220) (1:1500), anti-ATG-5 (Novus Biologicals, NB110-53818) (1:500), anti-Beclin 1 PAb (Novus Biologicals, NBP1-00088) (1:1000), anti-NF-κB Mab (1:1500) (Imgenex, IMG150B), antin-Lamin A/C MAb (Sigma, SAB4200236), anti-β-tubulin PAb (Abcam, ab6046) and anti-β-actin MAb (Sigma, A5316) (1:7500).

Techniques: Infection, Western Blot